‪Mads Berthinus Pedersen‬ - ‪Google Scholar‬

Biokemisk omvandling av lignocellulosa f3 centre

2013-1-21 · It is necessary to develop efficient methods to produce renewable fuels from lignocellulosic biomass. One of the main challenges to the industrialization of lignocellulose conversion processes is the large amount of cellulase enzymes used for the hydrolysis of cellulose. One method for decreasing the amount of enzyme used is to recycle the enzymes. Lignocellulosic biomass for bioethanol: an overview on pretreatment, hydrolysis and fermentation processes Rev Environ Health . 2019 Mar 26;34(1):57-68. doi: 10.1515/reveh-2018-0054.

', Journal of Chemical Technology and Biotechnology , vol. 92, no. 4, pp. 868-873. 2012-04-30 The cellulosomic saccharification of lignocellulose is affected by various substrate-related physical and chemical factors, including native (untreated) wood lignin content, the extent of lignin and xylan removal by pretreatment, lignin structure, substrate size, and of course substrate pore surface area or substrate accessibility to cellulose. Comparative study of corn stover pretreated by dilute acid and cellulose solvent-based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility. Biotechnology and Bioengineering 2009, 103 (4) , 715-724.

Optimization of the enzyme system for hydrolysis - OSTI.GOV

The acid hydrolysis of lignocellulosic material (LM) is one of the most widely studied and important subprocess in the LM biorefinery. After acid hydrolysis, LM can be converted to various biofuels, biochemicals, and biomaterials through chemical or biochemical methods.

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Also, lignocellulose solubilization by the ionic liquid allows processing at high concentrations, which can be a problem in enzymatic hydrolysis. On the other hand, our process improves on typical acid hydrolysis methods by avoiding the use of hazardous concentrated acid. In order to achieve efficient hydrolysis of lignocellulose, a series of enzymes are required to work in combination, which increases the cost of enzymes used in a biotechnological context. LIGNOCELLULOSE HYDROLYSIS 309 substance, slightly modified as the hemicellulose is removed, is termed lignin. Alternatively, lignin can be removed in a form that is considerably modified from the natural polymer by treatment with chlorine and leaching with sodium hydroxide (or by using sodium chlorite). Pulping and analytical methods of separation are discussed and a consideration is made of the potential products from lignocellulosic sources.

… Lignocellulosic biomass is currently available in large quantities as crop and forest residues or organic wastes, and could be produced at high yields by planting dedicated energy crops, but is not as easily processed by biochemical or thermochemical conversion technologies as other bio-based feedstocks such as sugars, starches, or oils. molecules, a process termed lignocellulose hydrolysis, remains one of the funda- mental challenges for agriculture (livestock feed), forestry (pulp and paper) and the energy industry (biofuels). 2016-1-1 · This article first discusses the structure of lignocellulose and factors that constrain the enzymatic hydrolysis of lignocellulosic materials, and then reviews the most commonly used pretreatment processes in improving the digestibility of lignocellulosic materials, with a special emphasis on combined pretreatment strategies. 2. Enhanced enzymatic hydrolysis of lignocellulose by optimizing enzyme complexes Appl Biochem Biotechnol. 2010 Mar;160(5):1407-14. doi: 10.1007/s12010-009-8602-3.
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Addition of surfactants to enzymatic hydrolysis of lignocellulose increases the conversion of cellulose into soluble sugars. The mechanism is not known for the increase of lignocellulose hydrolysis by surfactant hydrolysis phase, which can be performed by dilute acid, concentrated acid or enzymatically (Galbe & Zacchi, 2002). 4.1 Acid hydrolysis The main advantage of the acid hydrolysis is that acids can penetrate lignin without any preliminary pretreatment of biomass, … Reduction in the adsorption of cellulase onto lignin has been thought to be the common reason for the improvement of enzymatic hydrolysis of lignocellulose (EHLC) by a nonionic surfactant (NIS). Few research studies have focused on the relationship between lignocellulosic features and NIS for improving EHLC.

Enzyme derived from Trichoderma reesei have been utilised for lignocellulosic biomass hydrolysis. However, such enzymes are unable to efficiently hydrolyse hemicelluloses and lignin components in Thermostable enzymes offer potential benefits in the hydrolysis of lignocellulosic substrates; higher specific activity decreasing the amount of enzymes, enhanced stability allowing improved hydrolysis performance and increased flexibility with respect to process configurations, all leading to improvement of the overall economy of the process. Various pretreatment techniques change the physical and chemical structure of the lignocellulosic biomass and improve hydrolysis rates. During the past few years a large number of pretreatment methods have been developed, including alkali treatment, ammonia explosion, and others.
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Förbättrad lignocellulosahydrolys - Formas projektdatabas

show that the hydrolysis rate for the enzymes CBHI and EGI is reduced to Lignocellulosic biomass can be converted into fuels and chemicals using During enzymatic hydrolysis, the cellulose fibers and hemicellulose av R Wahlström · 2014 · Citerat av 6 — Total enzymatic hydrolysis of the polysaccharides in lignocellulosic biomass to monosaccharides is currently a focus research area. The monosaccharides Lignocellulosic biomass represents an attractive source of sugars for the furaldehyde inhibitors formed during the hydrolysis of lignocellulose solids contributions to high-solids enzymatic hydrolysis of lignocellulose w/w, indicating that inhibition of enzyme hydrolysis at lower solids concentrations is The bioreactor is used for cultivation of microorganisms (bacteria, yeast, and fungi) and enzymatic hydrolysis of lignocellulose. It is very flexible and can be used Improving the performance of enzymes in hydrolysis of high solids paper pulp derived from MSW. DJ Puri Method for the hydrolysis of lignocellulosic biomass. Large-scale bioethanol production from lignocellulosic biomass will require high solids loading in the enzymatic hydrolysis step.

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Mechanism of surfactant effect in enzymatic hydrolysis of

enzymatic hydrolysis, acid hydrolysis) the lignocellulosic (cellulose, hemicellulose, lignin) constituents of a sample are critical I'll first introduce you to cellulases, and then we'll look at a model of enzymatic hydrolysis of cellulose, and enzymes for hemicellulose and lignin. For cellulases 25 Apr 2017 Keywords: Second-generation bioethanol;. Lignocellulosic biomass; Cellulose and hemicellulose enzyme; Enzymatic hydrolysis. 1. Introduction.